Our laboratory is mainly interested in investigating the protein-protein interaction network and molecular regulatory mechanisms. Protein-protein interactions (PPIs) are crucial for almost all of the biological processes, including cell growth, proliferation, apoptosis etc. PPIs are also the important target of the development of drugs and target therapy in precision medicine. However, we still know little about the human interactome. Only less than 10% of human PPIs have been identified.
The research directions of our laboratory are as follows:
- We will develop automated, high-throughput and low-cost technologies for interactome research.
- To reveal the molecular mechanisms of interactome and identify the crucial targets for balancing, inhibiting and activating the PPIs, we will perform proteome-scale screening of the protein-protein interaction network and protein complexes of human liver or pathogen and host.
- To find the targets of disease prevention, treatment and molecular diagnosis, we will investigate the biochemical, cellular and molecular mechanisms of the liver cancer and immune diseases.
1. Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, et al. Toward an understanding of the protein interaction network of the human liver. Mol Syst Biol 2011, 7:536.
2. Wei J, Wei C, Wang M, Qiu X, Li Y, Yuan Y, Jin C, Leng L, Wang J#, Yang X#, He F#. The GTPase-activating protein GIT2 protects against colitis by negatively regulating Toll-like receptor signaling. Proc Natl Acad Sci U S A. 2014, 111(24):8883-8.
3. Yuan Y, Tian C, Gong Q, Shang L, Zhang Y, Jin C, He F#, Wang J#. An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein. J Proteome Res. 2015, 14 (1):154–163
4. Leng L, Xu C, Wei C, Zhang J, Liu B, Ma J, Li N, Qin W, Zhang W, Zhang C, Xing X, Zhai L, Yang F, Li M, Jin C, Yuan Y, Xu P, Qin J, Xie H, He F#, Wang J#. A Proteomics Strategy for the Identification of FAT10-Modified Sites by Mass Spectrometry. J Proteome Res. 2014, 13(1):268-76
5. Liu H, Yuan Y, Guo H, Mitchelson K, Zhang K, Xie L, Qin W, Lu Y, Wang J, Guo Y, Zhou Y, He F. Hepatitis B virus encoded x protein suppresses apoptosis by inhibition of the caspase-independent pathway. J Proteome Res. 2012, 11(10):4803-13.
6. Wang J, Yuan Y, Zhou Y, Guo L, Zhang L, Kuai X, Deng B, Pan Z,Li D, He F. A Protein Interaction Dataset Highlighted with Human Ras-MAPK/PI3K Signaling Pathways. J Proteome Res 2008, 7(9):3879-89
7. Zhang Y, Wang J#, Yuan Y, Zhang W, Guan W, Wu Z, Jin C, Chen H, Zhang L, Yang X#, He F#. Negative regulation of HDM2 to attenuate p53 degradation by ribosomal protein L26. Nucleic Acids Res 2010, 38(19):6544-6554.
8. Zhang W, Wang J#, Zhang Y, Yuan Y, Guan W, Jin C, Chen H, Wang X, Yang X#, He F#. The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1. Mol Biol Cell 2010, 21(14):2500-2513.
9. Yu M, Li H, Liu Q, Liu F, Tang L, Li C, Yuan Y, Zhan Y, Xu W, Li W, Chen H, Ge C, Wang J#, Yang X#. Nuclear factor p65 interacts with Keap1 to repress the Nrf2-ARE pathway. Cell Signal 2011, 23(5):883-892.